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Updated: Aug 10, 2026

Preparation of Quality Inositol Pyrophosphates
Published on: September 3, 2011
The structure and function of catalytic domains within inositol polyphosphate 5-phosphatases
J C Whisstock1, F Wiradjaja, J E Waters
1Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia. James.Whisstock@med.monash.edu.au
Abstract:
Phosphoinositide signaling pathways regulate many essential cellular functions including proliferation, differentiation and survival, cytoskeletal organization, and vesicular trafficking. The inositol polyphosphate 5-phosphatases regulate the cellular levels of several bioactive phosphoinositide species. This review describes the structure and function of the 5-phosphatase and Sac1 catalytic domains of these enzymes. The crystal structure of the 5-phosphatase domain has been solved and shares homology with members of the AP endonuclease family. The phosphoinositide polyphosphatase activity of the Sac1 domain, found in some inositol polyphosphate 5-phosphatases, is defined by a motif, CX5 R(T/S), also found in both protein and lipid phosphatases.
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