Related Experiment Video
Updated: Aug 5, 2026

08:30
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Published on: July 9, 2012
Correlation study of two routine bacteriology systems with previously characterised strains
A Giordano1, A Magni, C Graziani
1Department of Scienze e Sanità Pubblica University La Sapienza, Rome, Italy.
The New Microbiologica
|May 22, 2002
Summary
The VITEK 2 system accurately identifies antimicrobial resistance in Gram-positive and Gram-negative bacteria within 3-4 hours. It shows high agreement with the Sceptor system, aiding clinical antimicrobial susceptibility testing.
Area of Science:
- Clinical Microbiology
- Antimicrobial Resistance
- Bacterial Identification
Background:
- Accurate antimicrobial susceptibility testing is crucial for effective treatment of bacterial infections.
- Characterized bacterial strains with known resistance mechanisms are essential for performance evaluation of diagnostic systems.
Purpose of the Study:
- To evaluate the performance of the VITEK 2 system and its Advanced Expert System (AES) for antimicrobial testing.
- To compare the VITEK 2 system with the Sceptor system for routine antimicrobial susceptibility testing.
Main Methods:
- Antimicrobial susceptibility testing of characterized Gram-positive and Gram-negative bacterial strains using VITEK 2.
- Determination of Minimal Inhibitory Concentration (MIC) values by agar dilution.
- Comparison of VITEK 2 results with the Sceptor system.
Main Results:
- The VITEK 2 system demonstrated high agreement with the Sceptor system (91.4% and 88.2% overall).
- Agreement at specific antibiotic concentrations was higher for VITEK 2 (94%) compared to Sceptor (62%).
- The Advanced Expert System (AES) identified resistance mechanisms in 79.6% of strains.
Conclusions:
- The VITEK 2 system provides acceptable accuracy for antimicrobial sensitivity testing of diverse bacterial strains within 3-4 hours.
- The VITEK 2 system is a reliable tool for identifying antimicrobial resistance in clinical settings.
More Related Videos
Related Concept Videos
Microbial Classification System
Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...
Methods of Classification and Identification
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Applications of Molecular Taxonomy
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

