Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translational Regulation01:29

Translational Regulation

346
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
346
RNA Interference01:23

RNA Interference

27.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.1K
Experimental RNAi02:15

Experimental RNAi

6.8K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.6K
Types of RNA01:23

Types of RNA

70.8K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
70.8K
Types of RNA01:20

Types of RNA

8.2K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elucidating regulation of polyhydroxyalkanoate metabolism in Ralstonia eutropha: Identification of transcriptional regulators from phasin and depolymerase genes.

The Journal of biological chemistry·2024
Same author

Investigation of sRNA-mRNA Interactions in Bacillus subtilis In Vivo.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

In Vitro Methods for the Investigation of sRNA-mRNA Interactions in Bacillus subtilis.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Small proteins in Gram-positive bacteria.

FEMS microbiology reviews·2023
Same author

A comprehensive study of the interactions in the B. subtilis degradosome with special emphasis on the role of the small proteins SR1P and SR7P.

Molecular microbiology·2023
Same author

<i>Cis</i>- and <i>Trans</i>-Encoded Small Regulatory RNAs in <i>Bacillus subtilis</i>.

Microorganisms·2021

Related Experiment Videos

Antisense-RNA regulation and RNA interference.

Sabine Brantl1

  • 1Institut für Molekularbiologie, Friedrich Schiller Univ. Jena, Winzerlaer Str. 10, D-07745 Jena, Germany. Sabine.Brantl@rz.uni-jena.de

Biochimica Et Biophysica Acta
|May 22, 2002
PubMed
Summary
This summary is machine-generated.

RNA molecules are versatile, performing catalytic and regulatory roles beyond simple messaging. This review highlights antisense-RNA control and RNA interference (RNAi) mechanisms across life forms.

Related Experiment Videos

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Historically, RNA was viewed primarily as messenger RNA (mRNA), transfer RNA (tRNA), or ribosomal RNA (rRNA).
  • Emerging evidence reveals RNA's diverse functions, including catalytic activity (ribozymes) and regulation of biological processes like splicing and gene expression.
  • This review focuses on two key regulatory RNA functions: antisense-RNA control and RNA interference (RNAi).

Purpose of the Study:

  • To review the occurrence, biological roles, and mechanisms of antisense RNAs.
  • To summarize recent findings on RNA interference (RNAi), including involved genes and enzymes.
  • To discuss the intersection between antisense RNA control and RNAi.

Main Methods:

  • Literature review and synthesis of existing research on RNA function.
  • Comparative analysis of antisense RNA and RNAi mechanisms across different life forms.
  • Summarization of recent experimental findings and proposed models.

Main Results:

  • Antisense-RNA control is found in all three domains of life, with abundant examples in bacteria.
  • RNA interference (RNAi), a double-stranded RNA-triggered gene silencing mechanism, is an ancient antiviral system exclusive to eukaryotes.
  • Recent research has identified key genes and enzymes involved in RNAi pathways and elucidated its putative mechanisms.

Conclusions:

  • RNA molecules possess a broader range of functions than previously understood, including critical regulatory roles.
  • Antisense RNAs and RNAi represent significant gene regulatory mechanisms with distinct evolutionary histories and biological contexts.
  • Understanding these RNA-mediated processes provides insights into gene regulation, evolution, and antiviral defense.