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Thiocyanate utilization by an Arthrobacter.

P M Betts, D F Rinder, J R Fleeker

    Canadian Journal of Microbiology
    |November 1, 1979
    PubMed
    Summary
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    A soil bacterium, Arthrobacter, can use thiocyanate (SCN-) as a nitrogen source. This thiocyanate-degrading activity persists even with added nitrate or ammonium, indicating its robustness.

    Area of Science:

    • Microbiology
    • Environmental Science
    • Biochemistry

    Background:

    • Thiocyanate (SCN-) is an inorganic anion found in various environments.
    • Microbial degradation of thiocyanate is crucial for nitrogen cycling and detoxification.
    • Understanding microbial utilization of thiocyanate as a nitrogen source is important for bioremediation.

    Purpose of the Study:

    • To isolate and characterize soil microorganisms capable of utilizing thiocyanate (SCN-) as a sole nitrogen source.
    • To investigate the tolerance of the isolated organism to thiocyanate concentrations.
    • To determine the effect of alternative nitrogen sources on thiocyanate degradation.

    Main Methods:

    • Enrichment culture technique using thiocyanate (SCN-) as the nitrogen source.

    Related Experiment Videos

  • Isolation and identification of a bacterial species from soil.
  • Growth experiments with varying thiocyanate concentrations.
  • Assessing thiocyanate degradation by measuring 14CO2 release from SCN-(14C).
  • Supplementation studies with nitrate and ammonium ions.

    • Main Results:

    • An Arthrobacter species was successfully isolated, utilizing thiocyanate (SCN-) as a nitrogen source.
    • The isolate demonstrated tolerance to thiocyanate (SCN-) concentrations up to 0.1 M.
    • Thiocyanate (SCN-) degradation continued even when nitrate or ammonium ions were added to the culture.
    • Degradation activity was monitored by the release of 14CO2 from labeled thiocyanate (SCN-).
    • The thiocyanate (SCN-)-degrading activity decreased as the culture reached the stationary phase of growth.

    Conclusions:

    • Arthrobacter species can effectively utilize thiocyanate (SCN-) as a nitrogen source.
    • The thiocyanate (SCN-) degradation pathway in this isolate is robust and not significantly inhibited by common nitrogen sources.
    • This organism holds potential for bioremediation applications involving thiocyanate-contaminated environments.