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Architectural requirements for optimal activation by tandem CRP molecules at a class I CRP-dependent promoter
John Tebbutt1, Virgil A Rhodius, Christine L Webster
1School of Biosciences, University of Birmingham, Edgbaston, B15 2TT, UK.
Abstract:
The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase alpha subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase alpha subunit.