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[Soil strain of Bacillus subtilis harboring a large plasmid that mediates high-frequency conjugal mobilization]
O V Lotareva1, E U Poluéktova, M A Titok
1Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, Moscow, 113809 Russia.
Mikrobiologiia
|May 25, 2002
Abstract:
The ability of a soil strain of Bacillus subtilis harboring a large plasmid, p19, to mobilize a small staphylococcus plasmid, pUB110, was studied. The latter plasmid was transferred to the recipient cells of Bacillus subtilis 168 at a high frequency (about 10(-2) per recipient cell) both on filter surface and in liquid medium. Mobilization was initiated 40 to 50 min after the beginning of the contact between donor and recipient cells.