[Quantitative determination of N-terminal amino acids in fibrin by the Edman degradation method using thin-layer...

Separation and identification of phenylthiohydantoin derivatives (PTH) of 19 amino acids usually found in proteins was performed by thin-layer chromatography of "silufol" plates. Conditions of PTH quantitation were found at which a 90% extraction of PTH from silicagel was attained. A N-terminal analytical procedure for fibrin is elaborated, by which a high yield of N-terminal amino acids is obtained.