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Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples
1Institute for Medical Parasitology, Medical Faculty, University of Bonn, Germany. Panagiotis.Karanis@medizin.uni-koeln.de
Aims:
Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from tap water.
Methods And Results:
Ferric sulphate, aluminium sulphate and calcium carbonate were compared for their recovery efficiency of C. parvum oocysts from tap water. Lower mean recovery was achieved by calcium carbonate (38.8%) compared with ferric sulphate (61.5%) and aluminium sulphate (58.1%) for the recovery of 2.5 x 10(5) oocysts l(-1); 2.5 oocysts l(-1) and 1 oocyst l(-1) were adequately purified using ferric sulphate flocculation. In vitro excystation experiments showed that ferric sulphate flocculation does not markedly reduce the viability of oocysts.
Conclusions:
Ferric sulphate flocculation is a simple and effective tool for the purification of C. parvum oocysts from tap water.
Significance And Impact Of The Study:
The high recovery rates and low impact on oocyst viability provided by ferric sulphate flocculation might be useful for the detection of Cryptosporidium oocysts in environmental water samples.
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