Related Experiment Videos
Identification of a new HLA-B*15 allele, HLA-B*1569
David M Smillie1, P A Smith, S Day
1Histocompatibility Laboratory, National Blood Service, Sheffield, United Kingdom. david.smillie@nbs.nhs.uk
Tissue Antigens
|May 25, 2002
Abstract:
We have identified a new HLA-B*15 allele (B*1569) by polymerase chain reaction (PCR) using sequence-specific primers (SSP) and sequence-based typing (SBT). This novel allele was found in a 67-year-old white Caucasian male and differs from HLA-B*1503 at 3 positions. The nucleotide substitutions at positions 544, 559 and 560 result in amino acid changes at codon 158 from GCC (alanine) to ACC (threonine), and at codon 163 from CTG (leucine) to ACG (threonine).