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Mitochondrial malate dehydrogenase (Mor-1) in the mouse: linkage to chromosome 5 markers
Abstract:
Malate dehydrogenase is present in most mammalian tissues in both supernatant and mitochondrial forms. Although genetic variation for the supernatant form has not been observed in the mouse, electrophoretic variants caused by alleles at the mitochondrial locus (Mor-1) have been previously described. We have located this locus 11.0 +/- 2.9 cM from the beta-glucuronidase structural gene, Gus, on chromosome 5. The gene order is Hm-Pgm-1-rd-bf-Gus-Mor-1. Thus Mor-1 is presently the most distal marker on chromosome 5. Three different nuclear loci for mitochondrial enzymes (Mod-2, Got-2, and Mor-1) have now been mapped in the mouse, all on different chromosomes.
Insights
Researchers mapped the mitochondrial malate dehydrogenase locus (Mor-1) to mouse chromosome 5, identifying it as the most distal marker. This finding contributes to understanding genetic variation in mitochondrial enzymes across mammalian chromosomes.
Area of Science:
- Genetics and Genomics
- Biochemistry
- Molecular Biology
Background:
- Malate dehydrogenase exists in both supernatant and mitochondrial forms in mammalian tissues.
- Electrophoretic variants of the mitochondrial malate dehydrogenase locus (Mor-1) have been previously identified in mice.
- Genetic variation in the supernatant form of malate dehydrogenase has not been observed in mice.
Purpose of the Study:
- To genetically map the mitochondrial malate dehydrogenase locus (Mor-1) in the mouse.
- To determine the chromosomal location and order of the Mor-1 locus relative to other known genes.
Main Methods:
- Utilized genetic linkage analysis to determine the position of the Mor-1 locus.
- Established gene order on mouse chromosome 5 using known markers like beta-glucuronidase (Gus).
Main Results:
- The Mor-1 locus was mapped to mouse chromosome 5, located 11.0 +/- 2.9 centimorgans from the Gus gene.
- The determined gene order on chromosome 5 is Hm-Pgm-1-rd-bf-Gus-Mor-1.
- Mor-1 is identified as the most distal marker on mouse chromosome 5.
Conclusions:
- The Mor-1 locus has been successfully mapped to mouse chromosome 5.
- This mapping provides a new distal marker for chromosome 5, aiding future genetic studies.
- Three nuclear loci for mitochondrial enzymes (Mod-2, Got-2, and Mor-1) are now mapped to different mouse chromosomes.