Related Experiment Video

Updated: Oct 1, 2026

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
09:26

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Published on: February 20, 2015

Platelet-derived microparticles stimulate proliferation, survival, adhesion, and chemotaxis of hematopoietic cells

Monika Baj-Krzyworzeka1, Marcin Majka, Domenico Pratico

  • 1Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA.

Experimental Hematology
|May 29, 2002
PubMed
Abstract

Insights

Peripheral blood microparticles (PMPs) influence hematopoietic cell functions by transferring surface receptors. These PMPs chemoattract, increase adhesion, proliferation, and survival, impacting intercellular communication in hematopoiesis.

Area of Science:

  • Hematology
  • Cell Biology
  • Molecular Biology

Background:

  • Peripheral blood platelet-derived microparticles (PMPs) are circulating vesicles with potential to influence cellular functions.
  • Understanding PMP interactions with hematopoietic cells is crucial for comprehending hematopoiesis.

Purpose of the Study:

  • To investigate the effects of human PMPs on normal and malignant hematopoietic cells.
  • To characterize the surface molecules of PMPs and their role in cellular responses.

Main Methods:

  • Phenotyping of PMPs for surface molecule expression.
  • Assessing biological responses of CD34(+) stem/progenitor cells, precursor cells, and leukemic cell lines to PMPs.
  • Investigating intracellular signaling pathways activated by PMPs.

Main Results:

  • PMPs express various receptors including CXCR4, PAR-1, TNF receptors, CD95, CD40L, and PF-4.
  • PMPs transfer receptors to target cells, leading to chemoattraction, increased adhesion, proliferation, and survival.
  • PMPs activate intracellular signaling cascades (MAPK, PI-3K-AKT, STAT) in target cells; both protein and lipid components contribute to their activity.

Conclusions:

  • PMPs modulate biological functions of hematopoietic cells, suggesting a significant role in intercellular cross-talk.
  • Further research is required to identify specific PMP components responsible for distinct biological effects.

More Related Videos

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets08:50

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Published on: April 9, 2018

Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Production of Formed Elements01:34

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...