Related Experiment Video
Updated: Sep 13, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
[Nature of active sites during complex formation of DNA and Pt (II) compounds]
Abstract:
Quantitative estimation of the binding of Pt (II) with DNA and its derivatives is carried out and the selectivity of this reaction is studied. Absorption spectra and binding curves of Pt (II) with GC- and AT-enriched DNA fractions, apurinic and apyrimidinic acids, poly A and deoxyribonucleotides are studied. The strongest Pt (II) binding was observed in cytosine-containing nucleic acid components. The reduction of Pt (II) to Pt (O) took place only in the presence of cytosine. Adenine component was found to form 1 : 1 complex with chloroplatinit. A model of Pt (II) : DNA complex is proposed, in which a metal ion is bound with cytosine cycle through N3 atom. A complex is formed due to a high electron-acceptor capacity of cytosine cycle, the charge being transferred between platinum and DNA base. Thus, complex-bound platinum is capable of oxidating platinum ions in the solution.
More Related Videos
Related Concept Videos
Formation of Complex Ions
Ligand Binding and Linkage
DNA Helicases
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
Single-Strand DNA Binding Proteins
Nucleic Acid Structure
DNA Structure
DNA has a double-helix structure. The...

