[Effect of cryoprotectors on binding of bromthymol blue by bovine methemoglobin]
Iu V Kucherenko1, E D Rozanova
1Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkov.
Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
|May 31, 2002
Abstract:
Spectrophotometric method was used for study the binding of bromthymol blue dye (BTB) with bovine methemoglobin in 15% solutions of ethanol, glycerol and polyethylene glycol with molecular mass of 1.5 kDa (PEG-1500). It was shown, that adsorption of BTB by methemoglobin decreased in the sequence: glycerol > ethanol > PEG-1500. It is supposed that adsorption of the alcohols on the BTS sites of binding on methemolglobin led to the decrease of the amount of binding sites accessible for the dye.


