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Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
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[Cytotoxic effect of antineoplastic substances and their effect on DNA synthesis and cGMP level in tumor and normal

G P Pavlovskaia1, S S Tanina, N I Sharykina

  • 1Academy of Medical Science of Ukraine, Institute of Pharmacology and Toxicology, Kyiv, Ukraine.

Ukrains'Kyi Biokhimichnyi Zhurnal (1999 )
|May 31, 2002
PubMed

Insights

Several anticancer drugs effectively inhibit DNA synthesis, leading to a stable cytostatic effect against tumor growth. These agents also reduce cyclic guanosine monophosphate (cGMP) levels in certain tumors, indicating therapeutic potential.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Antitumor agents are crucial for cancer therapy.
  • Understanding their cytostatic mechanisms is vital for treatment efficacy.
  • The roles of DNA synthesis and cyclic guanosine monophosphate (cGMP) in tumor proliferation require further investigation.

Purpose of the Study:

  • To investigate the cytostatic effects of various antitumor agents.
  • To assess the impact of these agents on DNA synthesis and cGMP rates in both tumor and normal tissues.
  • To evaluate the stability and efficacy of these agents in different tumor models.

Main Methods:

  • Studied the effects of chlofiden, brotheophine, vincristine, adriablastine, natulan, and phthorafur.
  • Utilized three tumor models: Pliss lymphosarcoma, Yablonovskaya glioblastoma, and human glioblastoma heterografts.
  • Measured DNA synthesis rates and cyclic guanosine monophosphate (cGMP) levels.

Main Results:

  • Demonstrated that chlofiden, brotheophine, vincristine, adriablastine, natulan, and phthorafur significantly inhibit DNA synthesis.
  • Observed a marked and stable cytostatic effect and antitumor action with these agents.
  • Noted a decrease in cGMP rates in Pliss lymphosarcoma correlating with high antitumor activity.

Conclusions:

  • The studied antitumor agents exhibit significant DNA synthesis inhibition, leading to stable cytostatic and antitumor effects.
  • The observed decrease in cGMP levels in Pliss lymphosarcoma suggests its involvement in the mechanism of action of these drugs.
  • These findings support the therapeutic potential of these agents in cancer treatment.

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