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Increase in oxidative stress in kidneys of diabetic Akita mice
Yuki Ueno1, Fumihiko Horio, Koji Uchida
1Nagoya University Graduate School of Bioagricultural Sciences, Japan.
Abstract:
New biomarkers for oxidative damage, were used to identify whether hyperglycemia caused oxidative stress in diabetic Akita mice. At 13 weeks of age, the tissues of these mice were obtained, and the levels of N(epsilon)-(hexanonyl)lysine (HEL) and dityrosine (DT) were measured, these being related to lipid peroxide-derived protein covalent modification and protein cross-linking. The levels of HEL and DT in the kidneys of Akita mice were significantly increased compared with the control mice without any accumulation of thiobarbituric acid reactive substances and 4-hydroxy-2-nonenal-modified protein. Immunopositive staining was clearly observed in the kidneys of the Akita mice when using the anti-HEL antibody or anti-DT antibody. These results suggest that hyperglycemia in Akita mice induced oxidative stress and increased these markers in the kidneys.
Insights
Hyperglycemia in diabetic Akita mice induced oxidative stress, increasing kidney biomarkers N(epsilon)-(hexanonyl)lysine (HEL) and dityrosine (DT). These findings highlight new markers for oxidative damage in diabetes.
Area of Science:
- Biochemistry
- Diabetology
- Oxidative Stress Research
Background:
- Hyperglycemia is a hallmark of diabetes, often linked to increased oxidative stress.
- Identifying specific biomarkers of oxidative damage is crucial for understanding disease progression.
Purpose of the Study:
- To investigate whether hyperglycemia induces oxidative stress in diabetic Akita mice.
- To evaluate novel biomarkers, N(epsilon)-(hexanonyl)lysine (HEL) and dityrosine (DT), associated with oxidative damage.
Main Methods:
- Analysis of kidney tissues from 13-week-old diabetic Akita mice and control mice.
- Quantification of HEL and DT levels, indicators of lipid peroxide-derived protein modification and cross-linking.
- Immunohistochemical staining using anti-HEL and anti-DT antibodies.
Main Results:
- Significantly elevated levels of HEL and DT were observed in the kidneys of Akita mice compared to controls.
- No accumulation of thiobarbituric acid reactive substances or 4-hydroxy-2-nonenal-modified protein was detected.
- Immunopositive staining for HEL and DT was prominent in the kidneys of Akita mice.
Conclusions:
- Hyperglycemia in diabetic Akita mice leads to increased oxidative stress in the kidneys.
- HEL and DT serve as sensitive biomarkers for hyperglycemia-induced oxidative damage in diabetic kidneys.