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Related Experiment Videos

A prospective randomized study: day 2 versus day 5 embryo transfer.

Ingrid Van der Auwera1, Sophie Debrock, Carl Spiessens

  • 1Leuven University Fertility Center, University Hospital Gasthuisberg, Catholic University Leuven, Herestraat 49, B-3000 Leuven, Belgium. Ingrid.Vanderauwera@pandora.be

Human Reproduction (Oxford, England)
|June 4, 2002
PubMed
Summary

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Blastocyst transfers, where embryos are cultured to day 5/6, show higher clinical pregnancy rates per embryo transfer compared to day 2 transfers in IVF/ICSI patients. This suggests improved implantation success with later-stage embryo transfer.

Area of Science:

  • Reproductive Endocrinology
  • Embryology
  • In Vitro Fertilization (IVF)

Background:

  • The study investigates the efficacy of blastocyst transfer versus day 2 embryo transfer in an unselected in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) population.
  • The primary hypothesis tested is whether blastocyst transfers yield higher clinical pregnancy rates (CPR) per oocyte retrieval compared to day 2 transfers.

Purpose of the Study:

  • To compare the clinical pregnancy rates (CPR) per oocyte retrieval and per embryo transfer between blastocyst (day 5/6) and day 2 embryo transfers.
  • To evaluate implantation rates and cryo-augmented delivery rates in relation to embryo transfer timing.

Main Methods:

  • A randomized controlled study involving an unselected IVF/ICSI population.
  • Participants were blindly randomized to either day 2 (group 1) or day 5/6 (group 2) embryo transfer before ovarian stimulation.

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  • Oocytes and embryos were cultured using sequential media under controlled atmospheric conditions (5.5% CO2, 5% O2, 89.5% N2) with a maximum of two embryos transferred per patient.

    • Main Results:

    • Groups were comparable in terms of patient age, IVF indication, cycle history, fertilization rates, and number of embryos transferred.
    • Clinical pregnancy rate per embryo transfer was significantly higher in the blastocyst group (60%) compared to the day 2 group (35%) (P < 0.01).
    • Implantation rate per embryo transferred was also significantly higher in the blastocyst group (46%) versus the day 2 group (29%) (P < 0.03).

    Conclusions:

    • Blastocyst transfer results in a significantly higher clinical pregnancy rate per embryo transfer compared to day 2 transfer.
    • A trend towards a higher clinical pregnancy rate per oocyte retrieval was observed with blastocyst transfers, although not statistically significant in this study.
    • The findings support the benefit of extended embryo culture and blastocyst transfer for improving IVF/ICSI outcomes in unselected populations.