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Related Experiment Videos

CD-34 stromal expression pattern in normal and altered human corneas.

Paolo Toti1, Gian Marco Tosi, Claudio Traversi

  • 1Institute of Pathology, University of Siena, Siena, Italy. paolot@unisi.it

Ophthalmology
|June 5, 2002
PubMed
Summary

CD-34 immunoreactivity in corneal stromal cells is altered in diseases requiring penetrating keratoplasty. Loss of CD-34 expression is linked to scar formation and certain dystrophies, aiding in disease classification.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Immunohistochemistry

Background:

  • Corneal stromal cells, or keratocytes, play a crucial role in maintaining corneal transparency.
  • CD-34 is a cell surface glycoprotein expressed on various cell types, including some stromal cells.
  • Alterations in keratocyte markers may indicate disease processes affecting the cornea.

Purpose of the Study:

  • To investigate the expression of CD-34 in corneal stromal cells from normal and diseased corneas.
  • To correlate CD-34 immunoreactivity with specific pathologic conditions requiring penetrating keratoplasty.
  • To assess the diagnostic potential of CD-34 expression in corneal diseases.

Main Methods:

  • A prospective study analyzing 102 cornea buttons from patients undergoing penetrating keratoplasty.

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  • Control samples included healthy donor corneas and globes with intraocular neoplasms.
  • Immunohistochemistry was employed to examine CD-34 expression in stromal cells, alongside markers for endothelial cells (CD-31), leukocytes, and alpha-smooth muscle actin.

    • Main Results:

    • Normal keratocytes exhibited strong and consistent CD-34 immunoreactivity.
    • Diseases causing corneal opacity, such as keratoconus, herpes keratitis, trauma, and heredofamilial dystrophies, showed focal or diffuse loss of CD-34 expression.
    • Pseudophakic bullous keratopathy and Fuchs' endothelial dystrophy displayed mostly normal CD-34 immunoreactivity, with some cases showing patchy loss.

    Conclusions:

    • Loss of CD-34 immunoreactivity is associated with corneal scar formation (herpes keratitis, trauma) and may be a primary event in keratoconus and heredofamilial dystrophies.
    • Pseudophakic bullous keratopathy and Fuchs' endothelial dystrophy share similar CD-34 expression patterns, suggesting a potential grouping based on this marker.
    • CD-34 expression analysis offers insights into the cellular changes occurring in various corneal pathologies.