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A novel transactivating factor that regulates interferon-gamma-dependent gene expression.

Junbo Hu1, Qingjun Meng, Sanjit K Roy

  • 1Marlene and Stewart Greenebaum Cancer Center, Department of Microbiology and Immunology, Molecular and Cellular Biology Program, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.

The Journal of Biological Chemistry
|June 7, 2002
PubMed
Summary

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Researchers discovered a new interferon-gamma-activated transcriptional element (GATE) binding factor, GBF-1. This novel transcription factor plays a role in interferon signaling pathways and is present across multiple species.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • A novel interferon (IFN)-stimulated cis-acting enhancer element, gamma-IFN-activated transcriptional element (GATE), was previously identified.
  • GATE exhibits a unique primary sequence compared to known IFN-stimulated elements.
  • Preliminary findings suggest that novel transacting factors are required for the GATE-dependent transcriptional response.

Purpose of the Study:

  • To identify the transacting factors that bind to the GATE element.
  • To characterize a newly discovered factor involved in interferon signaling.

Main Methods:

  • Screening of a cDNA expression library from IFN-gamma-stimulated murine macrophages using a labeled GATE probe.
  • Identification and characterization of the GATE-binding factor-1 (GBF-1).

Related Experiment Videos

Main Results:

  • A novel transcription-activating factor, named GATE-binding factor-1 (GBF-1), was identified.
  • GBF-1 homologs are conserved across species including mouse, human, monkey, and Drosophila.
  • GBF-1 activates transcription from GATE-containing reporter genes, demonstrates strong transactivation but weak DNA binding, and its expression is induced by IFN-gamma.

Conclusions:

  • GBF-1 is a novel transactivating factor identified within interferon signaling pathways.
  • GBF-1 is expressed in various tissues and localized to both cytosolic and nuclear compartments.
  • The discovery of GBF-1 provides new insights into the molecular mechanisms of IFN signaling.