A more sensitive method for the quantitation of genomic DNA by Alu amplification
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Updated: Sep 30, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Published on: March 10, 2020
Melanie E Sifis1, Katrin Both, Leigh A Burgoyne
1Forensic Science Centre, Adelaide, SA, Australia.
Abstract:
Current procedures for human DNA quantitation reach their limit at 150 pg DNA, which is above the limit of the PCR profiling range using Profiler-Plus (Applied Biosystems, CA). This study tested the potential for the use of primate specific Alu sequences in forensic science for the sensitive detection and quantitaion of DNA. A fluorescently labelled primer pair was designed enabling high efficiency amplification of the core Alu sequence within primate DNA. Quantitation was performed by measurement of fluorescence intensity and comparison to a series of standard template DNA amounts via the construction of a standard curve. The new Alu-based quantitation protocol developed has shown its feasibility in more sensitively quantitating (100-2.5 pg) unknown amounts of human DNA for forensic use. The method is compatible with the use and throughput of current forensic procedures.
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