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A novel and rapid approach to isolating functional ryanodine receptors
Duncan J West1, Eileen C J Smith, Alan J Williams
1Department of Cardiac Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Dovehouse Street, London SW3 6LY, UK.
Biochemical and Biophysical Research Communications
|June 8, 2002
Abstract:
Conventional methods of isolating and reconstituting ryanodine receptors (RyRs) from native membranes into proteoliposomes take a minimum of 2 days to complete. We have developed an alternative strategy that can be used to isolate and reconstitute functional RyRs in just 3 h with a similar degree of purification. RyRs isolated by this method display characteristic functional behaviour as assessed by radioligand binding and single channel analyses.