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Automated acquisition of aptamer sequences.
J Colin Cox1, Manjula Rajendran, Timothy Riedel
1Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
Combinatorial Chemistry & High Throughput Screening
|June 8, 2002
Summary
Automated selection techniques can generate nucleic acid binding species (aptamers) for diverse protein targets. This automation integrates with analytical methods for efficient aptamer discovery and analysis.
Area of Science:
- Biotechnology
- Molecular Biology
- Biochemistry
Background:
- In vitro selection of aptamers involves complex liquid-handling steps.
- These steps are amenable to automation for increased efficiency.
Purpose of the Study:
- To demonstrate the capability of automated selection techniques for generating aptamers.
- To showcase the integration of automated aptamer selection with analytical methods.
Main Methods:
- Utilized automated liquid-handling systems for in vitro selection.
- Integrated automated sequencing, binding constant determination, and structural analysis.
- Explored multiplexing capabilities of the developed methods.
Main Results:
- Successfully generated aptamers against diverse protein targets using automated selection.
- Demonstrated seamless integration of automated selection with downstream analytical processes.
- Showcased the potential for multiplexed aptamer selection.
Conclusions:
- Automated aptamer selection is a viable and efficient method for generating aptamer binders.
- Automation significantly streamlines the aptamer discovery and characterization workflow.
- Future applications include selection against complex biological systems like proteomes and metabolomes.