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Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells
Erika Fernández-Vizarra1, Manuel J López-Pérez, José A Enriquez
1Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Miguel Servet, 177, Zaragoza 50013, Spain.
Methods (San Diego, Calif.)
|June 11, 2002
Abstract:
This article describes a quick basic method adapted for the purification of mammalian mitochondria from different sources. The organelles obtained using this protocol are suitable for the investigation of biogenetic activities such as enzyme activity, mtDNA, mtRNA, mitochondrial protein synthesis, and mitochondrial tRNA aminoacylation. In addition, these mitochondria are capable of efficient protein import and the investigation of mtDNA/protein interactions by DNA footprinting is also possible.