Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse model of optic neuropathy caused by mitochondrial complex I dysfunction.

Xian Zhang1, Dirk Jones, F Gonzalez-Lima

  • 1Institute for Neuroscience and Center for Molecular and Cellular Toxicology, University of Texas at Austin, Austin, TX 78712, USA.

Neuroscience Letters
|June 12, 2002
PubMed
Summary

Researchers created a mouse model for optic neuropathy by inhibiting mitochondrial complex I. This model mimics human mitochondrial optic neuropathy and can accelerate testing of new treatments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interhemispheric Metabolic Effects of Transcranial Infrared Laser Stimulation of the Prefrontal Cortex.

Human brain mapping·2026
Same author

Abdominal Photobiomodulation and the Gut-Brain Axis: A Systematic Review of Mechanistic and Translational Evidence.

Biomedicines·2025
Same author

Photobiomodulation mechanisms: duration of action in the human prefrontal cortex.

Frontiers in behavioral neuroscience·2025
Same author

Machine Learning Prediction of the Cognitive Responses to Transcranial Prefrontal Photobiomodulation in Individuals With Bipolar Disorder Using Functional Near-Infrared Spectroscopy.

IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society·2025
Same author

Augmenting Internet-Based Cognitive Behavioral Therapy for Major Depressive Disorder With Transcranial Infrared Laser Stimulation.

Biological psychiatry global open science·2025
Same author

Cognitive improvement and prefrontal network interactions in individuals with remitted bipolar disorder after transcranial infrared laser stimulation.

Frontiers in psychiatry·2025

Area of Science:

  • Neuroscience
  • Ophthalmology
  • Mitochondrial Biology

Background:

  • Mitochondrial dysfunction is implicated in neurodegenerative diseases.
  • Optic neuropathies, such as Leber's hereditary optic neuropathy, affect vision.
  • A suitable animal model is needed to study these conditions and test therapies.

Purpose of the Study:

  • To develop a novel mouse model of optic neuropathy.
  • To investigate the effects of mitochondrial complex I inhibition on retinal structure.
  • To establish a preclinical tool for evaluating treatments for mitochondrial optic neuropathies.

Main Methods:

  • Induction of optic neuropathy via intravitreal injection of rotenone (a complex I inhibitor) in CBA/J mice.
  • Histochemical staining of retinal sections to assess complex I enzymatic activity.

Related Experiment Videos

  • Measurement of retinal thickness, specifically the retinal ganglion cell layer, at various time points post-injection.

    • Main Results:

    • Significant thinning of the retinal ganglion cell layer was observed in rotenone-injected eyes compared to controls.
    • Maximum thinning of 43% occurred at 24 hours post-injection.
    • The observed retinal changes are consistent with degeneration seen in human optic neuropathies.

    Conclusions:

    • This study successfully established the first mouse model of optic neuropathy due to mitochondrial complex I dysfunction.
    • The model effectively replicates key pathological features of mitochondrial optic neuropathies.
    • This model provides a valuable platform for rapid screening of potential therapeutic interventions for mitochondrial neurodegenerative diseases.