Characterization of interaction between protein and carbohydrate using CZE
Related Experiment Video
Updated: Sep 30, 2026

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
Published on: December 23, 2022
Mohamed Al-Arhabi1, Yahya Mrestani, Heinrich Richter
1Institute of Pharmaceutical Chemistry, Martin-Luther-University, Wolfgang-Langenbeck-Str. 4, D-06120 Halle, Germany.
Abstract:
The present study describes the application of the capillary zone electrophoresis (CZE) to investigate interactions between Concanavalin A (Con A) and the one of their major components (L-asparagin) with a variety of carbohydrates (D-glucose, D-fructose, D-mannose D-galactose, maltose and lactose). It was observed that the carbohydrates show different interactions with Con A and L-asparagin. Addition of carbohydrates to the electrolyte buffer and different concentrations of carbohydrates led to a change of the migration time and the ionic mobility of Con A and L-asparagin. A mathematical model for quantitative evaluation to calculate the aggregation constants (K) was used. Our investigations show that it is possible to characterize the interaction between carbohydrates and proteins or amino acid quantitatively using CZE.
More Related Videos
08:53Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
14:44A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
Published on: September 24, 2012
Related Concept Videos
Protein-protein Interfaces
Protein-Protein Interfaces
Chemistry of Carbohydrates
Chemistry of Carbohydrates
Chemistry of Carbohydrates
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...