Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural centrosome aberrations favor proliferation by abrogating microtubule-dependent tissue integrity of breast epithelial mammospheres.

Oncogene·2015
Same author

Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2013
Same author

Centriole overduplication through the concurrent formation of multiple daughter centrioles at single maternal templates.

Oncogene·2007
Same author

Centrosome cohesion is regulated by a balance of kinase and phosphatase activities.

Journal of cell science·2001
Same author

Cloning and characterization of Plx2 and Plx3, two additional Polo-like kinases from Xenopus laevis.

Experimental cell research·2001
Same author

The mitotic roles of Polo-like kinase.

Journal of cell science·2001
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 21, 2013

The centrosome cycle.

P Meraldi1, E A Nigg

  • 1Department of Cell Biology, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany.

FEBS Letters
|June 18, 2002
PubMed
Summary
This summary is machine-generated.

The centrosome, crucial for cell division and structure, must duplicate and segregate with chromosomes. Its cycle regulation by phosphorylation and proteolysis is key to preventing diseases like cancer.

More Related Videos

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Related Experiment Videos

Last Updated: Jun 26, 2026

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 21, 2013

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • The centrosome acts as the primary microtubule-organizing center in animal cells.
  • Centrosome function is vital for maintaining cell shape, polarity, and forming the mitotic spindle.
  • Aberrations in centrosome number or structure are linked to diseases, particularly cancer.

Purpose of the Study:

  • To review recent findings on the regulation of the centrosome cycle.
  • To explore the coordination between the centrosome cycle and the chromosomal cell cycle.
  • To highlight the role of phosphorylation and proteolysis in these processes.

Main Methods:

  • Literature review of recent research on centrosome cycle regulation.
  • Synthesis of information on molecular mechanisms controlling centrosome duplication and segregation.
  • Analysis of the interplay between centrosome and chromosome cell cycles.

Main Results:

  • The centrosome cycle involves precise duplication and segregation synchronized with chromosome behavior.
  • Phosphorylation and proteolysis are critical regulatory mechanisms governing centrosome structural and functional transitions.
  • Dysregulation of the centrosome cycle is associated with pathological conditions, including cancer.

Conclusions:

  • Understanding centrosome cycle regulation is essential for comprehending cell division fidelity.
  • The coordination with the chromosomal cell cycle ensures proper cell proliferation.
  • Further research into these regulatory pathways may offer therapeutic targets for diseases involving centrosome abnormalities.