Molecular dynamics simulations revealed Ca(2+)-dependent conformational change of Calmodulin
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Updated: Sep 30, 2026

Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
Yuto Komeiji1, Yutaka Ueno, Masami Uebayasi
1IMCB/RICS, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba, Japan. y-komeiji@aist.go.jp
Abstract:
Molecular dynamics simulations were performed to simulate Ca(2+)-dependent conformational change of calmodulin (CaM). Simulations of the fully Ca(2+)-bound form of CaM (Holo-CaM) and the Ca(2+)-free form (Apo-CaM) were performed in solution for 4 ns starting from the X-ray crystal structure of Holo-CaM. A striking difference was observed between the trajectories of Holo-CaM and Apo-CaM: the central helix remained straight in the former but became largely bent in the latter. Also, the flexibility of Apo-CaM was higher than that of Holo-CaM. The results indicated that the bound Ca(2+) ions harden the structure of CaM.
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