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An integrated microfabricated cell sorter.

Anne Y Fu1, Hou-Pu Chou, Charles Spence

  • 1Department of Applied Physics, California Institute of Technology, Pasadena 91125, USA.

Analytical Chemistry
|June 19, 2002
PubMed
Summary
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Researchers created an automated microfluidic cell sorter using soft lithography. This device precisely manipulates and sorts cells, demonstrating effective recovery of Escherichia coli bacteria on-chip.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Sorting

Background:

  • Microfluidic devices offer precise control over biological samples.
  • Automated cell sorting is crucial for various biological and medical applications.
  • Integrated systems reduce complexity and improve efficiency in cell manipulation.

Purpose of the Study:

  • To develop an integrated microfluidic cell sorter.
  • To incorporate multiple microfluidic functionalities for automated cell sorting.
  • To demonstrate precise cell manipulation and sorting capabilities.

Main Methods:

  • Utilized multilayer soft lithography for microfabrication.
  • Integrated peristaltic pumps, dampers, and switch valves for fluid control.
  • Implemented cell trapping algorithms and optical interrogation for analysis.

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Main Results:

  • Achieved an active valve volume as small as 1 picoliter.
  • Optical interrogation volume was approximately 100 femtoliters.
  • Successfully sorted and recovered Escherichia coli cells on the microfluidic chip.

Conclusions:

  • The developed integrated cell sorter enables coordinated and automated cell sorting.
  • Microfluidic functionalities allow for precise cell manipulation and analysis.
  • Demonstrated the potential of the device for bacterial cell sorting and recovery.