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Introduction of functional groups onto polypropylene and polyethylene surfaces for immobilization of enzymes
Azmi Naqvi1, Pradip Nahar, R P Gandhi
1Centre for Biochemical Technology (CSIR), Delhi University Campus, Mall Road, Delhi, 110007, India.
Analytical Biochemistry
|June 19, 2002
Abstract:
Polypropylene and polyethylene surfaces are activated by introducing an active functional group through 1-fluoro-2 nitro-4-azidobenzene by UV irradiation. Horseradish peroxidase and glucose oxidase are immobilized onto the activated surfaces, simply by incubating the enzymes at 37 degrees C. When untreated surfaces are used, insignificant immobilization of the enzymes is observed.