Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple astrocyte responses to lysophosphatidic acids.

Marion R Steiner1, Jan R Urso, Jennifer Klein

  • 1Department of Microbiology and Immunology, MS-415 Medical Center, University of Kentucky, Lexington, KY 40536, USA. mrstei01@pop.uky.edu

Biochimica Et Biophysica Acta
|June 19, 2002
PubMed
Summary

Lysophosphatidic acid (LPA) plays diverse roles in the brain, affecting astrocytes and glioma cells. LPA influences calcium, morphology, migration, and gene expression, with responses varying by brain region.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations Between Socioeconomic Status and Adult Congenital Heart Disease Mortality in the United States: Insights From the Global Burden of Disease Study.

Journal of the American Heart Association·2026
Same author

Early Outcomes of Nurse-Led Outreach for Lost to Follow-Up Patients after Pediatric Heart Surgery.

European journal of cardiovascular nursing·2026
Same author

Operational performance of the 2023 World Heart Federation screening criteria for rheumatic heart disease in a high-risk, resource-limited population.

Open heart·2026
Same author

Impact of a patient-facing video on CHG bathing compliance in patients admitted to a bone marrow transplant unit.

Infection control and hospital epidemiology·2026
Same author

Identifying Inadequate Maternal Nutrition in Pregnancies Affected by Fetal Heart Defects: A Feasibility Pilot Study Using Photo-Based Diet Quality Assessment.

Journal of cardiovascular development and disease·2026
Same author

Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing.

Frontiers in bioengineering and biotechnology·2026

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Lysophosphatidic acid (LPA) and its receptors are prevalent in the brain.
  • Their levels change during brain development and injury, indicating significant roles.
  • LPA signaling is implicated in various neurological processes.

Purpose of the Study:

  • To investigate the multifaceted roles of LPA in the brain, particularly in astrocyte and glioma cell functions.
  • To understand how LPA affects cellular responses like calcium concentration, morphology, and migration.
  • To explore LPA's impact on astrocyte signaling pathways and gene expression.

Main Methods:

  • Cultured astrocytes and glioma-derived cells were utilized.
  • Cellular responses including intracellular calcium concentrations, morphological changes, and migration were measured.

Related Experiment Videos

  • Key signaling events such as reactive oxygen species synthesis, protein kinase activation, and gene expression (c-fos, c-jun) were analyzed.

    • Main Results:

    • LPA induced significant changes in intracellular calcium levels and morphology in cultured cells.
    • LPA stimulated glioma cell migration and reactive oxygen species synthesis in astrocytes.
    • Astrocyte responses to LPA, including proliferation and cytokine expression, varied by brain region, species, and developmental stage.
    • Micromolar LPA concentrations were required for some responses, such as cytokine stimulation and glutamate uptake inhibition, relevant to brain pathologies.

    Conclusions:

    • LPA signaling is a critical regulator of astrocyte and glioma cell behavior in the brain.
    • The context-dependent nature of LPA responses highlights its complex role in neurological functions and pathologies.
    • Further research is needed to fully elucidate LPA's functions in brain development, astrocyte activation, and intercellular communication.