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Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR)
Gehua Wang1, Connie Barton, Frank G Rodgers
1National Laboratory for Enteric Pathogens, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2.
Journal of Microbiological Methods
|June 19, 2002
Abstract:
For RT-PCR, removal of contaminating genomic DNA in RNA samples using manganese sulfate was more effective than magnesium. DNA contamination was removed in 3 microg of nucleic acid using 10 U of RNase-free DNase I in 10-microl reaction volumes. The digestion procedure was compatible with commercial RNA extraction kits and was suitable for RT-PCR assay.