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Updated: Aug 24, 2026

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 21, 2010
Structural determination of the O-chain polysaccharide from Agrobacterium tumefaciens, strain DSM 30205
Cristina De Castro1, Olga De Castro, Antonio Molinaro
1Dipartimento di Chimica Organica and Biochimica, Università di Napoli, Italy. decastro@unina.it
Abstract:
Agrobacterium tumefaciens is a Gram-negative, phytopathogenic bacterium and is characterized by an unique mode of action on dicotyledonous plants: it is able to genetically modify the host, and because of this feature, it is used as a tool for transgenic plants. Many experiments have demonstrated that lipopolysaccharides (LPSs) play an important role for the disease development, as they are involved in the adhesion process of the bacterium on the plant cell wall. Despite the wealth of information on the role of LPS on phytopathogenesis, the present paper appears as the first report on the molecular primary structure of the O-chain produced from Agrobacterium. Its repeating unit was determined by means of chemical and spectroscopical analysis, and has the following structure: (3)-alpha-D-Araf-(1-->3)-alpha-l-Fucp-(1-->.
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