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Purification of glutathione S-transferase fusion proteins from yeast

Avital A Rodal1, Mara Duncan, David Drubin

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

Methods in Enzymology
|June 21, 2002
PubMed
Abstract

No abstract available in PubMed .

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...