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Changes of plasma free choline and choline-containing compounds' concentrations and choline loss during hemodialysis
Yesim Ozarda Ilcol1, Kamil Dilek, Mustafa Yurtkuran
1Department of Biochemistry, Uludag University Medical School, Bursa, Turkey.
Objectives:
This study was undertaken to determine the changes in plasma free choline and choline-containing compounds in end stage renal disease (ESRD) and to determine if they were lost into the dialysate during hemodialysis.
Design And Methods:
Plasma and dialysate free choline, phosphocholine and phospholipid-, phosphatidylcholine-, sphingomyelin-bound choline were measured before, during and after hemodialysis.
Results:
Plasma free and bound choline concentrations (mean +/- standard error of the mean) were 12.9 +/- 0.6 and 2697 +/- 57 microM or 37.3 +/- 0.9 and 2792 +/- 98 microM in controls or in ESRD patients, respectively. Free choline concentrations were correlated (r = 0.598; p < 0.001) with the time the patients were subjected to hemodialysis. Plasma free choline and phosphocholine concentrations are decreased by a total of -8.1 +/- 0.6 micromol/L and -88 +/- 8 micromol/L, respectively; phospholipid-, phosphatidylcholine- and sphingomyelin-bound choline are increased, during hemodialysis. Patients lost about 350 micromoles of choline into the dialysate during hemodialysis.
Conclusion:
Plasma free choline concentrations are elevated in ESRD, and a considerable amount of choline is lost into the hemodialysate.
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