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Updated: Jul 30, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
Published on: April 20, 2015
Membrane proteins: shaping up
Chen-Ni Chin1, Gunnar von Heijne, Jan-Willem L de Gier
1Department Molecular Biophysics and Biochemistry, Yale University, Bass 429, 266 Whitney Avenue, New Haven, CT 06520, USA.
Abstract:
Over recent years, much progress has been made in the identification and characterization of factors involved in the biosynthesis of integral membrane proteins of the helix-bundle type. In addition, our knowledge of membrane protein structure and the forces stabilizing helix-helix interactions in a lipid environment is expanding rapidly. However, it is still not clear how a membrane protein folds into its final form in vivo, nor what constraints there are on the folded structure that results from the mechanistic details of translocon-mediated assembly rather than simply from the thermodynamics of protein-lipid interactions.
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