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Updated: Aug 15, 2026

Expression Analysis of Mammalian Linker-histone Subtypes
Published on: March 19, 2012
[Sequencing and homology analysis of cDNAs encoding FhGST and AsGST]
1Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Aim:
To study the homologies between FhGST, AsGST and Sj26GST.
Methods:
Total RNAs were extracted from Fasciola hepatica (Fh) and Ascaris suum (As) by guanidine hydrochloride/CsCl ultra-centrifugation. Two pairs of primers were designed according to FhGST and AsGST sequences. RT-PCR was carried out using total RNA as template. PCR products were directly sequenced. Then the homology analysis was made using DNASIS software on nucleotide level.
Results:
A FhGST403 bp fragment and a AsGST411 bp fragment were obtained and they both located in the open reading frame.
Conclusion:
On nucleotide level, the homologies are 59% and 54% between the two cloned fragments and Schistosoma japonicum 26 kDa GST (Sj26GST) respectively.
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