Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular methods for exploring the intestinal ecosystem.

G W Tannock1

  • 1Department of Microbiology, University of Otago, Dunedin, New Zealand. gerald.tannock@stonebow.otago.ac.nz

The British Journal of Nutrition
|June 29, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Eczema-protective probiotic alters infant gut microbiome functional capacity but not composition: sub-sample analysis from a RCT.

Beneficial microbes·2018
Same author

A functional analysis of the formyl-coenzyme A (frc) gene from Lactobacillus reuteri 100-23C.

Journal of applied microbiology·2014
Same author

Coliforms and enterococci isolated from the intestinal tract of conventional mice.

Microbial ecology·2013
Same author

Association of rat, pig, and fowl biotypes of lactobacilli with the stomach of gnotobiotic mice.

Microbial ecology·2013
Same author

Mini review: Molecular genetics: A new tool for investigating the microbial ecology of the gastrointestinal tract?

Microbial ecology·2013
Same author

Treatment and secondary prevention effects of the probiotics Lactobacillus paracasei or Bifidobacterium lactis on early infant eczema: randomized controlled trial with follow-up until age 3 years.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2011
Same journal

Gut Microbiome and Environmental Enteric Dysfunction Are Unchanged by 18 Months of Small-Quantity Lipid-Based Nutrient Supplementation among Young Children in Lusaka, Zambia.

The British journal of nutrition·2026
Same journal

The Effect of Asparagus Stem Consumption on Health Outcomes in Adults: A Systematic Review.

The British journal of nutrition·2026
Same journal

Adherence to a Healthy Plant-Based Diet is Associated with Adiposity Indicators in Costa Rican Adults.

The British journal of nutrition·2026
Same journal

The Relationship between Food Literacy and Dietary Intake and Diet Quality in Adults: A Cross-Sectional Study.

The British journal of nutrition·2026
Same journal

Diet quality and risk of using medication for hypertension up to 11 years after delivery: Testing the predictive validity of a proposed revision of the Nutri-Score and the current Nutri-Score in the NewTools project.

The British journal of nutrition·2026
Same journal

Within- and Between-Day Reliability of Laboratory-Based Body Composition Assessment Methods.

The British journal of nutrition·2026
See all related articles

Analyzing intestinal microflora composition using molecular methods like PCR and DGGE helps identify disease-associated bacterial populations. This research paves the way for targeted interventions with probiotics or prebiotics to restore gut health.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Gastroenterology

Background:

  • Intestinal microflora plays a crucial role in maintaining health and disease states.
  • Understanding the composition of gut bacteria is essential for diagnosing and treating various conditions.
  • Molecular techniques offer advanced tools for microbial community analysis.

Purpose of the Study:

  • To explore the utility of molecular methods for analyzing intestinal microflora.
  • To compare bacterial communities in numerous samples efficiently.
  • To identify altered bacterial populations linked to specific diseases.

Main Methods:

  • Utilized polymerase chain reaction (PCR) combined with denaturing gradient gel electrophoresis (DGGE).
  • Applied these molecular techniques for high-throughput comparison of bacterial communities.

Related Experiment Videos

  • Focused on analyzing samples from both healthy and diseased states.

    • Main Results:

    • Demonstrated the efficiency and effectiveness of PCR-DGGE for comparing complex bacterial communities.
    • Enabled the identification of distinct bacterial populations associated with disease states.
    • Provided a foundation for further investigation into disease-specific microbial alterations.

    Conclusions:

    • Molecular methods, particularly PCR-DGGE, are powerful tools for studying intestinal microflora.
    • Identifying abnormal bacterial populations is key to understanding gut dysbiosis.
    • Future therapeutic strategies may involve probiotics and prebiotics to modulate gut microbiota.