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Validation of Vitek version 7.01 software for testing staphylococci against vancomycin.

P M Raney1, P P Williams, J E McGowan

  • 1Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. pfr7@cdc.gov

Diagnostic Microbiology and Infectious Disease
|June 29, 2002
PubMed
Summary
This summary is machine-generated.

The Vitek automated system shows improved sensitivity for detecting staphylococci with reduced vancomycin susceptibility. However, it may shift minimum inhibitory concentration (MIC) data towards higher values, generally not impacting categorical interpretations.

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Area of Science:

  • Clinical microbiology
  • Antimicrobial resistance

Background:

  • Vancomycin is a critical antibiotic for treating Staphylococci infections.
  • Accurate susceptibility testing is essential for effective treatment.
  • Automated systems are increasingly used for microbial identification and susceptibility testing.

Purpose of the Study:

  • To compare vancomycin Minimum Inhibitory Concentration (MIC) results generated by the National Committee for Clinical Laboratory Standards broth microdilution (BMD) reference method with those from the Vitek automated system (version 7.01).
  • To evaluate the performance of the Vitek system in detecting staphylococci with reduced vancomycin susceptibility.

Main Methods:

  • 143 staphylococcal isolates were tested.
  • Vancomycin MICs were determined using the BMD reference method.
  • Vitek automated system (GPS-105 and GPS-107 cards, version 7.01 software) was used for comparison.

Main Results:

  • Vitek showed improved sensitivity in detecting reduced vancomycin susceptibility compared to earlier versions.
  • Vitek results exhibited a shift towards higher vancomycin MICs for some isolates.
  • Vitek did not report MICs of 1 or 4 µg/mL, reporting values of < or =0.5 µg/mL or 2-16 µg/mL instead.
  • Retesting of two isolates with high Vitek MICs (> or =32 µg/mL) yielded lower MICs (< or =0.5 µg/mL) on Vitek, while BMD showed 1 µg/mL.

Conclusions:

  • The Vitek system (version 7.01) demonstrates enhanced sensitivity for detecting staphylococci with reduced vancomycin susceptibility.
  • Laboratories may observe a shift in MIC data towards higher values using Vitek, though categorical interpretations are largely unaffected.
  • Discrepancies in MIC values, particularly at lower concentrations, warrant attention for accurate susceptibility reporting.