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Updated: Sep 30, 2026
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
Mutation detection of the human glucocorticoid receptor alpha gene area coding for the hormone-binding domain by
A F Tsolakidou1, S A Coulocheri, C E Sekeris
1Laboratory of Biological Chemistry, University of Athens Medical School, 75, Mikras Asias Str., GR-115 27, Athens, Greece.
Abstract:
Mutations in the hormone-binding domain of the human glucocorticoid receptor alpha (hGRalpha) gene have been detected in a variety of glucocorticoid resistance syndromes. Using the denaturing gradient gel electrophoresis technique, we developed a sensitive method for the detection of alterations in the gene area coding for the whole hormone-binding domain and part of the DNA-binding domain of the hGRalpha. This method can be applied for screening of glucocorticoid receptor gene alterations in glucocorticoid-dependent diseases.
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