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Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells
Tohru Saeki1, Noriki Takahashi, Ryuhei Kanamoto
1Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University, Japan. tsaeki@kpu.ac.jp
Abstract:
The mouse Na+/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [3H]taurocholic acid with Km and Vmax values of 18 microM and 102 pmol/mg protein/min, respectively. This Km value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [3H]taurocholic acid transport.
