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Green fluorescent protein-labeled recombinant fluobody for detecting the picloram herbicide
In Seon Kim1, Jae Han Shim, Yong Tack Suh
1Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Canada. ikim@ualberta.ca
Abstract:
A green fluorescent protein-labeled fluobody was designed to develop a simple immunoassay method for detecting picloram herbicide in an environmental sample. The gfp gene was successfully inserted into the pSJF2 vector harboring the picloram-specific antibody fragment to yield pSJF2GFP. Picloram spiking in an environmental river sample could be indirectly detected by observing the fluorescence intensity value of the gfp-fluobody, exhibiting specific sensitivity to free picloram with an IC50 value of 50 ppb. Using the gfp-fluobody immunoassay avoids the enzyme-substrate reaction for calorimetric detection that is required in an enzyme-linked immunosorbent assay (ELISA).