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Microbial production of extra-cellular phytase using polystyrene as inert solid support.

Pinky Gautam¹, A Sabu, Ashok Pandey

¹Biotechnology Division, Council of Scientific and Industrial Research, Trivandrum, India.

Bioresource Technology

|July 4, 2002

Summary

This study shows Aspergillus ficuum and Rhizopus oligosporus can produce phytase using polystyrene in solid-state fermentation (SSF). Optimized conditions yielded significant enzyme production, demonstrating polystyrene

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Area of Science:

Microbiology
Biotechnology
Enzyme Technology

Background:

Phytase enzymes are crucial for phosphorus bioavailability.
Solid-state fermentation (SSF) is an efficient method for enzyme production.
Inert supports can influence microbial growth and enzyme yield in SSF.

Purpose of the Study:

To investigate the potential of using polystyrene as an inert support for phytase production.
To optimize SSF conditions for maximal phytase yield by Aspergillus ficuum and Rhizopus oligosporus.
To evaluate the efficacy of polystyrene in supporting microbial phytase synthesis.

Main Methods:

Solid-state fermentation (SSF) using Aspergillus ficuum TUB F-1165 and Rhizopus oligosporus TUB F-1166.
Polystyrene was utilized as the inert support material.
Optimization of fermentation parameters including substrate pH, moisture content, incubation temperature, and inoculum size.
Enzyme activity was quantified as U/g dry substrate (U/gds).

Main Results:

Both A. ficuum and R. oligosporus produced extracellular phytase on polystyrene supports.
Maximal phytase production for A. ficuum was 10.07 U/gds under specific optimized conditions (pH 6.0, 58.3% moisture, 30°C, 1.3 x 10^7 spores/5 g, 72h).
Maximal phytase production for R. oligosporus was 4.52 U/gds under different optimized conditions (pH 7.0, 58.3% moisture, 30°C, 1 x 10^6 spores/5 g, 96h).

Conclusions:

Polystyrene is a viable and effective inert support for microbial phytase production via SSF.
Optimized fermentation parameters are critical for maximizing phytase yield from A. ficuum and R. oligosporus.
This study highlights a novel application of polystyrene in biotechnological enzyme production.