Susceptibility testing of Aspergillus spp. by means of an automated blood culture system
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Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry
Published on: May 24, 2024
P-M Rath1, J M Freise, R Ansorg
1Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Hufelandstrasse 55, D-45122 Essen, Germany. rath@uni-essen.de
Abstract:
The BacT/Alert blood culture system was evaluated for the MIC determination of amphotericin B for 27 strains of Aspergillus from five different species. An inoculum of c. 10(8) cfu/mL and an incubation time of 48 h resulted in MICs for 26 of 27 strains that corresponded with published MICs obtained in a microdilution assay. Twelve of 13 strains with MIC(48) values >/=2 mg/L showed growth within 24 h in bottles containing 0.25 mg/L amphotericin B, indicating that results can be obtained the day following inoculation. It is concluded that the BacT/Alert blood culture system can be used for susceptibility testing of Aspergillus spp. with amphotericin B.
