[Transformation of glucose-1-phosphate by hemolysates of sheep erythrocytes]

Transformation of glucose-1-phosphate (G-1-P) by hemolyzates of sheep erythrocytes was studied depending on its concentration, dilution of erythrocytes, manganese ions and time of incubation. MgC12 in a concentration of 5-25 mM is shown to produce a two-fold intensification of G-1-P transformation and formation of fructose. A further increase in MgC12 concentration results in a gradual and at last, complete inhibition of the phosphoglucomutase reaction. A two-fold rise in the content of erythrocyte hemolyzate protein increases respectively the utilization of G-1-P. Transformation is 4 mM and time of incubation 60 min. The sum of sugars in the process of incubation does not change essentially, that evidences for inconsiderable transformation of G-1-P up to lactic acid. An increase in the pentoses content is observed with low concentrations of the substrate only.