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Serotyping and RAPD profiles of Salmonella enterica isolates from Mauritius
M H R Khoodoo1, M I Issack, Y Jaufeerally-Fakim
1Faculty of Agriculture, University of Mauritius, Réduit, Mauritius. mhrk@altern.org
Letters in Applied Microbiology
|July 9, 2002
Summary
Salmonella strains from chickens and humans in Mauritius show genetic similarities, indicating potential transmission routes. A specific DNA marker was identified for the Salmonella genus.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- The genus Salmonella is a frequent cause of gastroenteritis in Mauritius.
- Salmonella infections are more prevalent during summer months compared to winter.
Purpose of the Study:
- To evaluate the genetic diversity of Salmonella enterica isolates from Mauritius using RAPD fingerprinting.
- To investigate the genetic relationship between human and chicken Salmonella isolates.
Main Methods:
- Twenty-six bacterial isolates were collected from local hospital laboratories and poultry producers.
- Random Amplified Polymorphic DNA (RAPD) fingerprinting was performed on 24 confirmed Salmonella isolates (19 human, 5 chicken) using four primers.
- Biochemical and serological analyses were conducted, alongside cluster analysis using NTSYS-pc software.
Main Results:
- Two initial chicken isolates were misidentified; 24 isolates were confirmed as Salmonella.
- RAPD analysis generated 70 markers, revealing genetic diversity among the isolates.
- Cluster analysis indicated genetic similarities between certain Salmonella strains isolated from humans and chickens.
Conclusions:
- Genetic similarities exist between human and chicken Salmonella strains in Mauritius, suggesting potential transmission.
- A specific 1 kbp DNA band, amplified by primer OPA-10, was identified as a marker for the Salmonella genus.