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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

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Related Experiment Video

Updated: Jul 7, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
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An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis.

U Albufera1, P Bhugaloo-Vial, M I Issack

  • 1Agricultural and Food Sciences Department, University of Mauritius, Reduit, Mauritius.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
|February 5, 2008
PubMed
Summary

Molecular typing of Salmonella using REP-PCR and RAPD distinguished human and food isolates. REP-PCR offered superior discriminatory power, correlating well with serogrouping and clustering patterns.

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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

Area of Science:

  • Microbiology
  • Molecular Biology
  • Food Safety

Background:

  • Salmonella is a significant foodborne pathogen.
  • Differentiating Salmonella strains from various sources is crucial for public health surveillance and outbreak investigations.

Purpose of the Study:

  • To characterize and differentiate Salmonella isolates from human and food sources using molecular typing techniques.
  • To compare the discriminatory power of REP-PCR and RAPD for Salmonella strain typing.

Main Methods:

  • Conventional culture, biochemical, and serological analyses were performed.
  • DNA fingerprinting was achieved using Repetitive Extragenic Palindromic PCR (REP-PCR) with ERIC, REP, and BOXAIR primers.
  • Random Amplified Polymorphic DNA (RAPD) analysis was conducted using OPP-16 and OPS-11 primers.
  • Dendrogram and Principal Coordinate Analysis (PCA) were used for data interpretation.

Main Results:

  • REP-PCR demonstrated higher discriminatory power and produced more complex banding patterns than RAPD.
  • Molecular profiles generally separated human and non-human (food) Salmonella isolates.
  • A strong correlation was found between serogrouping (O antigen) and molecular profiles.
  • Two human isolates showed close relatedness to some non-human isolates.

Conclusions:

  • REP-PCR is a robust method for differentiating closely related Salmonella strains.
  • Molecular typing effectively distinguishes Salmonella isolates from human and food origins.
  • Combined serological and molecular data provide a comprehensive approach for Salmonella characterization.