Automated PCR/sequence template purification
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Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
Published on: April 19, 2013
1Department of Microbiology, Stobhill Hospital, Glasgow, UK. stuart.clarke@northglasgow.scot.nhs.uk
Molecular Biotechnology
|July 10, 2002
Abstract:
A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.

