Related Experiment Video
Updated: Jun 27, 2026

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019
Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi
Zi-Xu Wang1, Qiu-Jun Mo1, Jun-Wei Xu2
1Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
Abstract:
The ATP-citrate lyase gene (acl) was cloned and overexpressed in Ganoderma lingzhi. Overexpression of acl increased the accumulation of ganoderic acids (GAs) in G. lingzhi in both liquid shake culture and solid-state fermentation (SSF) conditions, with higher production observed in the SSF. In the SSF, the maximum contents of individual GAs (GA-Mk, GA-T, GA-S, and GA-Me) and total GAs in the acl-overexpressing G. lingzhi (ACL strain) reached 107.65 ± 3.19, 301.82 ± 27.38, 152.65 ± 15.28, and 259.77 ± 23.42 μg/100 mg dry weight (DW) and 9.47 ± 0.61 mg/100 mg DW, respectively, representing 1.42-, 1.74-, 2.01-, 1.85-, and 1.72-fold higher than those of the wild-type (WT) strain. The ACL strain also showed elevated ACL enzyme activity (145.23 ± 8.81 U/g) and acetyl-CoA content (74.85 ± 3.23 μg/g), which were 2.08 and 2.82 times greater than those of the WT strain, respectively. Additionally, the maximum levels of the key intermediates squalene and lanosterol were improved by 1.77- and 1.54-fold, respectively, and transcript levels of the genes encoding 3-hydroxy-3-methylglutaryl coenzyme A enzyme (hmgr), squalene synthase (sqs), and lanosterol synthase (ls) were upregulated by 3.46-, 7.21-, and 5.85-fold, respectively, compared with those in the WT strain. Collectively, these results demonstrate that overexpression of the acl is an effective strategy for enhancing GA biosynthesis in G. lingzhi.
Related Concept Videos
Cell Specific Gene Expression
GPCRs Regulate Adenylyl Cylase Activity
Two...
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
ATP Synthase: Mechanism
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
