[Determination of fluoride in bone samples from human iliac crest with an ion-specific electrode (author's transl)]
Abstract:
A simple potentiometric method for the determination of fluoirde ion in human bones is described. The bone samples were taken from a definite position in the iliac crest. The samples are prepared for analysis by ashing at 550 degrees C, dissolving in HNO3 (4 mol/1) and adjusting the pH with NaOH (4 mol/1) and Total Ion Strength Adjustment Buffer, Absolute values are derived from a calibration curve based on aqueous NaF-buffer solutions. The calibration curve is linear in the range 5.2-10(-3)-1.0-10(-5) mol/1F-. The minimum limit of measurement is 5.2 X 10(-6) mol/1F-. The method has an accuracy of 96.1 % or 92.3% with a series precision based on the Pearson variability coefficient of 1.68%. The normal mass fraction of fluoride in human bones was found to be between 69 and 1740 - 10(-6) F-/ash.
