Radiation- and chemical-induced structural chromosome aberrations in human spermatozoa
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Updated: Sep 30, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Yujiroh Kamiguchi1, Hiroyuki Tateno
1Department of Biological Sciences, Asahikawa Medical College, 2-1-1-1 Midorigaoka-higashi, Asahikawa, Japan. yujiroh@asahikawa-med.ac.jp
Abstract:
Previous studies on the clastogenic effects of mutagens on human sperm chromosomes were reviewed. A marked increase of structural chromosome aberrations (SCAs) has been reported in the spermatozoa irradiated in vitro with five kinds of ionizing radiation (137Cs gamma-, 60Co gamma-, X-, and 3H beta-rays and 252Cf neutrons). The micronucleus (MN) test with hybrid two-cell embryos generated from human sperm and hamster oocytes was shown to be useful as a simple and rapid method for assessing the effects of radiation. Radiosensitivity of human spermatozoa was highest, being followed by golden hamster, Chinese hamster and mouse spermatozoa. Chromosome-damaging effects were also found with some chemicals (bleomycin, daunomycin, methyl methanesulfonate, triethylenemelamine, neocarzinostatin, N-methyl-N'-nitro-N-nitorosoguanidine and mitomycin C (MMC)), but not with other chemicals (urethane, nitrobenzene, dioxin, cyclophosphamide (CP), benzo(a)pyrene (BP) and N-nitrosodimethylamine (NDMA)). The clastogenicity of chemical metabolites was confirmed for CP and BP, by using the S9-based metabolic activation system. The results of sperm chromosome analysis from cancer patients who had undergone radio- and/or chemotherapy were contradictory among investigators and further studies are necessary. The importance of mutagenicity testing with human spermatozoa is discussed.
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