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Thin layer chromatographical detection of tyrosine produced from L-[U-(14)C]phenylalanine by ruminal microbes
R I Khan1, R Onodera, M R Amin
1Department of Animal Science, Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. rikhanah@bdonline.com
Abstract:
Thin layer chromatographical detection of tyrosine (Tyr) synthesized from L-[U-(14)C]phenylalanine (Phe) (1 mM) by rumen bacteria (B) and protozoa (P) collected from fistulated Japanese Goat was carried out. About 16 and 12% of the added Phe was converted to Tyr by B and P, respectively. Large amount of radioactivity in ether fractions indicated an abundant production of aromatic acids from Phe. Small amount of radioactivity found in CO(2) fractions implied an occurrence of considerable decarboxylation reaction(s) by rumen bacteria and protozoa.
Insights
Rumen bacteria and protozoa convert phenylalanine to tyrosine. Aromatic acids were also produced, with some evidence of decarboxylation reactions occurring.
Area of Science:
- Microbiology
- Biochemistry
- Animal Science
Background:
- Rumen microorganisms play a crucial role in nutrient metabolism in ruminants.
- Understanding the metabolic pathways of amino acids in the rumen is essential for animal nutrition.
Purpose of the Study:
- To investigate the synthesis of tyrosine from phenylalanine by rumen bacteria and protozoa.
- To quantify the conversion rates and identify other metabolic products.
Main Methods:
- Thin layer chromatography was used for the detection of tyrosine.
- Radioactive labeling (L-[U-(14)C]phenylalanine) was employed to trace metabolic conversions.
- Rumen bacteria and protozoa were collected from fistulated Japanese goats.
Main Results:
- Approximately 16% of phenylalanine was converted to tyrosine by rumen bacteria, and 12% by protozoa.
- Significant radioactivity was detected in ether fractions, indicating abundant production of aromatic acids.
- Radioactivity in CO(2) fractions suggested decarboxylation reactions by rumen microorganisms.
Conclusions:
- Rumen bacteria and protozoa possess the metabolic capability to synthesize tyrosine from phenylalanine.
- The metabolism of phenylalanine in the rumen involves the production of aromatic acids and decarboxylation reactions.